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bioRxiv · 10.1101/2025.08.09.669357

Cold exposure induces the constitutively active thermogenic receptor, GPR3, via ERRα and ERRγ

Abstract

ObjectivesDespite transformative advances in obesity pharmacotherapy, safely increasing energy expenditure remains a key unmet need. Exploiting thermogenic adipocytes represents a promising target given their capacity for significant catabolic activity. We previously showed that G protein-coupled receptor 3 (GPR3) can drive energy expenditure in brown and white mouse and human adipocytes. GPR3 is a unique GPCR because it displays high intrinsic activity and leads to constitutive cAMP signaling upon reaching the cell surface. Therefore, the transcriptional induction of GPR3 is analogous to ligand-binding activation of most GPCRs. Gpr3 expression is physiologically induced in thermogenic adipocytes by cold exposure and mimicking this event through overexpression in mice is fully sufficient to increase energy expenditure and counteract metabolic disease. Yet the factors mediating physiological Gpr3 expression remain unknown. MethodsHere, we apply ATAC-Seq to identify cold-induced promoter elements of Gpr3. We uncover a role for the estrogen-related receptors, ERR and ERR{gamma}, in the physiological transcriptional control of Gpr3 using adipose-specific double knock-out mice with and without adeno associated virus (AAV)-mediated rescue. ResultsWe show that ERR directly binds the cold-induced promoter element of Gpr3 and that adipocyte ERR and ERR{gamma} are required for the in vivo transcriptional induction of Gpr3 during cold exposure. Importantly, deficient Gpr3 cold-inducibility in adipose-specific ERR and ERR{gamma} KO mice is fully rescued by delivery of AAVs re-expressing either ERR or ERR{gamma} directly into brown adipose tissue. ConclusionsERR and ERR{gamma} are critical regulators of cold-induced transcription of Gpr3 and represent a targetable strategy for pharmacologically unlocking GPR3-induced energy expenditure.

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Sveidahl Johansen, O., McIntyre, R. L., Rahbani, J., Scholtes, C., Lagarde, D. M., Tandio, D., Linde Basse, A., Giguere, V., Kazak, L., Gerhart-Hines, Z.. 2025-08-09. Cold exposure induces the constitutively active thermogenic receptor, GPR3, via ERRα and ERRγ. https://doi.org/10.1101/2025.08.09.669357

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