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bioRxiv · 10.1101/2025.08.08.669389

Aging-Associated Microbiota Drives Treg Dysfunction via TNF Signaling

Abstract

Aging is associated with a chronic, low-grade inflammatory state referred to as inflammaging, which contributes to impaired immune regulation and increased susceptibility to disease. While regulatory T (Treg) cells are key mediators of immune homeostasis, their role in the context of age-related inflammation remains poorly understood. Here we demonstrate that age-related changes in the microbiota promote impaired Treg cell function, resulting in the differentiation of inflammatory T cells. In agreement, we find that aged germ-free (GF) mice exhibited a more balanced immune profile, where the Treg cells are functional and pro-inflammatory mediators are reduced, suggesting that microbial exposure is essential for the establishment of inflammaging. Furthermore, we show that the use of old microbiota in young animals was sufficient to induce pro-inflammatory T cell responses and impaired mucosal Treg cell proliferation, while young microbiota restored Treg cell function in old animals. Mechanistically, we show that exposure to aged microbiota was associated with sustained TNF signaling, elevated oxidative stress, DNA damage, and increased expression of senescence markers such as {gamma}H2AX and p16 in Treg cells. These findings uncover a microbiota-TNF-dependent mechanism by which age-associated microbial dysbiosis drives Treg cell dysfunction and promotes immune aging, highlighting the therapeutic potential of microbiota-targeted strategies to restore immune homeostasis in the elderly.

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BibTeXRIS

Leite, J. A., Ergun, Z., Stylianakis, E., Jasser, R., Ingelfinger, F., Bos, N., Zonouzi, A. P., Mark, M., Carter, K., Liu, X., Hoevelmeyer, N., Regen, T., Pasparakis, M., Neumann, C., Amit, I., Yosef, N., Reinhardt, C., Waisman, A.. 2025-08-11. Aging-Associated Microbiota Drives Treg Dysfunction via TNF Signaling. https://doi.org/10.1101/2025.08.08.669389

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