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bioRxiv · 10.1101/2025.08.07.669117

Real-time, High-throughput Super-resolution Microscopy via Panoramic Integration

Yoon, K.·Yoon, H.·Tadesse, K.·Ling, Z.·Mandracchia, B.·Datta, S.·Bozdag, G. O.·Burnetti, A. J.·Ratcliff, W. C.·Jia, S.
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Abstract

We introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized line-scan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity. This versatile platform offers a practical pathway toward biological insights beyond traditional optical and computational constraints.

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Yoon, K., Yoon, H., Tadesse, K., Ling, Z., Mandracchia, B., Datta, S., Bozdag, G. O., Burnetti, A. J., Ratcliff, W. C., Jia, S.. 2025-08-11. Real-time, High-throughput Super-resolution Microscopy via Panoramic Integration. https://doi.org/10.1101/2025.08.07.669117

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