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bioRxiv · 10.1101/2025.08.04.668485

Reversible therapeutic resistance in EGFR mutant lung cancer caused by RB1 loss induced lineage plasticity

Abstract

Treatment with EGFR tyrosine kinase inhibitors (EGFR TKI) is standard of care for patients with lung cancer initiated by activating mutations in the EGFR gene. While EGFR TKI treatment is effective, virtually all patients progress on therapy. Loss of function mutations in the RB1 tumor suppressor gene are associated with poor EGFR TKI outcomes, but underlying mechanisms remain unclear. Analysis of genetically engineered experimental models and human clinical specimens described here demonstrates RB1 loss has minimal impact on initial cell cycle responses to EGFR TKI but markedly accelerates acquired resistance over time. RB1 loss promotes transcriptional plasticity that enables rare cells to spontaneously assume reversible, resistance-conferring lineage states. These findings highlight the importance of non-genetic adaptations in driving acquired EGFR TKI resistance, advance our understanding of how RB1 impacts cancer, and identify opportunities to improve EGFR TKI treatment outcomes by targeting reversible epigenetic resistance mechanisms.

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Zhang, L., Chinnam, M., Balk, B., Zhang, X., Wang, Y., Shaurova, T., Alam, S., Pokharel, S., Singh, P., Jacobi, J., Wang, J., Cortes Gomez, E., Habitzruther, M., Seshadri, M., Atanassov, B., Wu, W., Benevolenskaya, E., Blakely, C. M., Hershberger, P., Long, M., Bivona, T., Goodrich, D. W.. 2025-08-06. Reversible therapeutic resistance in EGFR mutant lung cancer caused by RB1 loss induced lineage plasticity. https://doi.org/10.1101/2025.08.04.668485

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