Purification of amylase from local isolate Bacillus subtilis A4

Awda, J.; Fayyadh, A. H.

doi:10.1101/2025.08.04.668472

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bioRxiv · 10.1101/2025.08.04.668472

Purification of amylase from local isolate Bacillus subtilis A4

Awda, J.·Fayyadh, A. H.

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Abstract

The amylase produced from local isolate Bacillus subtilis A4 was purified by precipitation with 80-25% saturation ammonium sulphate, followed by ion-exchange chromotography using DEAE-cellulose column, and Gel filtration using Sephdex G-100 column. In the stratification step, one peak of the enzyme was observed in the ion exchange and three major protein peaks in the recovered parts and the second summit was a container on enzymatic efficacy only the other peaks were completely empty. The number of purification times was 6 times and with an enzyme yield of 49%.

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Awda, J., Fayyadh, A. H.. 2025-08-04. Purification of amylase from local isolate Bacillus subtilis A4. https://doi.org/10.1101/2025.08.04.668472

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