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bioRxiv · 10.1101/2025.07.27.667088

Discovery of evolutionarily extended cis-regulatory overlapping genes expanding the protein universe from animals to humans

Nomura, Y.·Dohmae, N.
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Abstract

A recent proteogenomics-driven approach has uncovered actively translated alternative open reading frames (altORFs) that complement reference protein-coding sequences (refCDSs). Here, we further explored proteogenomic data to identify hidden proteomes, specifically short ORF-encoded polypeptides (SEPs) and longer altORF-encoded proteins (LEPs) previously overlooked in human genomes. We discovered a novel class of proteomes originating from SEP/LEP-coding upstream overlapping altORFs (oORFs) associated with refCDSs, which cis-regulate refCDS translation and have undergone evolutionary selection favoring C-terminal extension for the enhanced cis-regulation. These translatable oORFs occur in signal effectors of the Hippo-YAP/TAZ, p53, Wnt, and TGF-{beta} crosstalk pathways and frequently arise from intragenic frameshift polymorphisms closely linked to human diseases. The intragenic frameshift mutations divide refCDSs and capture the N-terminal regions to form the new oORFs. Consequently, we termed these entities upstream region-usurping repurposed proteins (USURPs). These findings offer new insights into birth and evolution of proteomes, broadening our understanding of the protein universe influenced by genome dynamics. TeaserUnveiling a novel category of proteomes from animals to humans and elucidating its birth, evolution, function, and relation to human diseases

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Nomura, Y., Dohmae, N.. 2025-07-31. Discovery of evolutionarily extended cis-regulatory overlapping genes expanding the protein universe from animals to humans. https://doi.org/10.1101/2025.07.27.667088

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