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bioRxiv · 10.1101/2025.07.26.666927

Haplotype-resolved T2T gap-free genomes of the winegrape cultivar 'Cabernet Sauvignon'

Khan, F. S.·Sun, T.·Wang, X.·Zhang, X.·Abe-Kanoh, N.·Su, Y. H.·Guo, L.·Ye, W.
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Abstract

Cabernet Sauvignon (CS), a cultivar of winegrape (Vitis vinifera), is among the most renowned winegrape varieties globally. In this study, we released the haplotype-resolved telomere-to-telomere (T2T) CS genome assembled using a combination of PacBio HiFi, ONT ultra-long, and Hi-C sequencing data. The two T2T gap-free haplotype-resolved assemblies CS-T2T.Hap1 and CS-T2T.Hap2 sized 491.11 Mb (contig N50 = 25.09 Mb) and 491.90 Mb (contig N50 = 25.51 Mb), respectively. Genome annotation predicted a total of 36,456 genes in CS-T2T.Hap1 and 35,471 genes in CS-T2T.Hap2. By genome comparison, we discovered and validated megabase inversion events on Chromosome 03,11,18 and 19, which are not prevalent in other haplotype-resolved V. vinifera genomes. In summary, this haplotype-resolved T2T genome represents an essential genomic resource for Cabernet Sauvignon, and lays the foundation for its genetic studies, improvement and utilization.

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Khan, F. S., Sun, T., Wang, X., Zhang, X., Abe-Kanoh, N., Su, Y. H., Guo, L., Ye, W.. 2025-07-27. Haplotype-resolved T2T gap-free genomes of the winegrape cultivar 'Cabernet Sauvignon'. https://doi.org/10.1101/2025.07.26.666927

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