A sublethal drought and rewatering time course reveals intricate patterning of responses in the annual Arabidopsis thaliana

Abstract

Drought is a major factor of yield loss in annual crops. It triggers a wide range of physiological and molecular changes which are mediated by a suite of transcription factors from at least four different protein families. In the literature, the observed phenotypic changes and the number of transcriptome changes upon drought in Arabidopsis thaliana varies widely. To resolve the apparent variation, we conducted a phenotyping and transcriptomics experiment with progressive drought which is initially mild and escalates to strong but still sublethal drought followed by rewatering. Phenotypic data is analyzed with machine learning methods and connected to transcriptome data. The phenotypic data show that drought stress manifests in distinct stages. The transcriptional analysis shows one threshold program and gradual expression programs among typical drought responsive regulons over time and during recovery. Plant aging prior to senescence and the drought response overlap to a large degree and drought stressed plants rejuvenate transcriptionally before returning to the control aging program. The phenotypic traits are associated with different transcript abundances again reflecting multiple overlaying programs. Transcripts with high explanatory power of phenotypes are biomarkers and not causal for the phenotype. Significance statementLarge scale phenotyping and transcriptomics during progressive drought demonstrate that the drought response in the annual Arabidopsis thaliana is controlled by multiple transcriptional programs, mostly with gradual onset, that overlap with the plant aging program. Transcripts with high explanatory power for phenotypes are biomarkers and not causal.