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bioRxiv · 10.1101/2025.07.18.665522

Multi-parameter spectral flow cytometry panel for immune phenotyping of murine B and T cell responses

Hoetzel, K.·Feuerstein, H.·Ludwig, J.·Wardemann, H.
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Abstract

Graphical abstractTo enable the analysis of B cell and T cell immune responses in mouse lymph nodes, spleen, and bone marrow, including different B cell and T cell subsets and their activation status, as well as the antigen-reactivity and isotype of B cells and level of T cell exhaustion, a novel 31-parameter spectral flow cytometry panel was developed. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=199 SRC="FIGDIR/small/665522v1_ufig1.gif" ALT="Figure 1"> View larger version (52K): org.highwire.dtl.DTLVardef@1efbad2org.highwire.dtl.DTLVardef@1d23e4borg.highwire.dtl.DTLVardef@1f7d3caorg.highwire.dtl.DTLVardef@17177e2_HPS_FORMAT_FIGEXP M_FIG C_FIG Created in https://BioRender.com

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Hoetzel, K., Feuerstein, H., Ludwig, J., Wardemann, H.. 2025-07-18. Multi-parameter spectral flow cytometry panel for immune phenotyping of murine B and T cell responses. https://doi.org/10.1101/2025.07.18.665522

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