Contrasting needle physiological strategies to soil nutrient scarcity in radiata pine plantations

Ruiz de Larrinaga, L.; San Miguel-Oti, F.; Monasterio, A.; Artetxe, U.; Munne-Bosch, S.; Mesa, T.; Moreaux, C.; Ortega-Barrueta, U.; Sertutxa, U.; Pena, L.; Ametzaga, I.; Yuste, J. C.; Esteban, R.

doi:10.1101/2025.07.15.664982

bioRxiv · 10.1101/2025.07.15.664982

Contrasting needle physiological strategies to soil nutrient scarcity in radiata pine plantations

Ruiz de Larrinaga, L.·San Miguel-Oti, F.·Monasterio, A.·Artetxe, U.·Munne-Bosch, S.·Mesa, T.·Moreaux, C.·Ortega-Barrueta, U.·Sertutxa, U.·Pena, L.·Ametzaga, I.·Yuste, J. C.·Esteban, R.

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Abstract

Intensive European forestry practices contribute to soil degradation and nutrient depletion, compromising tree health and ecosystem stability. However, the influence of soil nutrient scarcity on tree physiological responses during drought remains unclear, particularly in Pinus radiata D. Don plantations, where it may impair needle function and overall tree health. We investigated how soil nutrient availability influences leaf-level physiological strategies during drought by comparing same-aged needles from two P. radiata stands with contrasting management stages and ages: (i) trees ({approx}20 years), likely to be clear-cut at 30-35 years (herein managed), and (ii) trees (>45 years) not clear-cut at the typical rotation age (herein abandoned). During the severe summer drought of 2022, both stands exhibited downregulation of the photosynthetic apparatus, indicating impaired photosynthetic performance under drought. However, their leaf-physiological responses to nutrient scarcity diverged. Managed trees exhibited dependence on soil nutrients, with reduced photosynthetic performance under nutrient-poor conditions. In contrast, abandoned trees showed relative independence from soil nutrients, maintaining photosynthetic function even under nutrient-poor conditions. This highlights a trade-off: younger, managed trees may enhance photosynthesis under optimal nutrient conditions but are more susceptible to nutrient imbalances and environmental stress. In turn, older, abandoned trees appeared to buffer the effects of drought and nutrient scarcity through age-related physiological traits. Our findings underscore the physiological value of mature stands. Furthermore, higher soil organic carbon and vegetation diversity in abandoned stands suggest that management cessation may enhance long-term ecosystem resilience. These results emphasise the importance of integrating soil-leaf interactions into sustainable forest management.

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Ruiz de Larrinaga, L., San Miguel-Oti, F., Monasterio, A., Artetxe, U., Munne-Bosch, S., Mesa, T., Moreaux, C., Ortega-Barrueta, U., Sertutxa, U., Pena, L., Ametzaga, I., Yuste, J. C., Esteban, R.. 2025-07-19. Contrasting needle physiological strategies to soil nutrient scarcity in radiata pine plantations. https://doi.org/10.1101/2025.07.15.664982

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