Growth phase-specific gene regulation and algicidal interactions between a new A. macleodii strain and the model diatom T. pseudonana

Abstract

Phytoplankton-bacteria interactions are pivotal in marine ecosystems, influencing primary production and biogeochemical cycles. Diatoms, in particular, engage in diverse relationships with bacteria, ranging from mutualism to pathogenicity. However, the mechanisms governing the shift between these interactions and how they are shaped by host physiology and environmental context, remain unclear. To address this, we investigated how the diatom growth phase influences the interaction between a newly isolated Alteromonas macleodii strain from the Equatorial Pacific and the model diatom Thalassiosira pseudonana. We demonstrated that A. macleodiis algicidal activity depends on the diatoms growth phase, defensive capacity, and substrate availability. The algicidal effect manifests either during the diatoms stationary phase or with an external source of organic carbon, implicating organic matter availability as a key driver. Transcriptomic analysis revealed that A. macleodii shifts from motility-associated to growth-associated gene expression patterns in response to the diatoms growth phase and co-culture duration. Filtrate assays and fluorescence microscopy suggest a two-stage infection model: initial bacterial motility and exudate secretion induce diatom death, followed by bacterial aggregation around cellular debris. Comparative transcriptomics of A. macleodii with other algal hosts highlights host-specific bacterial responses, underscoring the context-dependent nature of these interactions. Together, these findings reveal how bacterial behavior and gene expression are modulated by host state and environmental cues, providing a molecular basis for the dynamic roles of diatom-bacteria interactions in shaping microbial community structure.