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bioRxiv · 10.1101/2025.07.09.663992

Resolving Emergent Oscillations in Gene Circuits with a Growth-Coupled Model

Abstract

Synthetic gene circuits often behave unpredictably in batch cultures, where shifting physiological states are rarely accounted for in conventional models. Here, we find that degradation-tagged protein reporters could exhibit transient oscillatory expression, which standard single-scale models do not capture. We resolve this discrepancy by developing Gene Expression Across Growth Stages (GEAGS), a dual-scale modeling framework that explicitly couples intracellular gene expression to logistic population growth. Using a chemical reaction network (CRN) model with growth-phase-dependent rate-modifying functions, GEAGS accurately reproduces the observed transient oscillations and identifies amino acid recycling and growth-phase transition as key drivers. We reduce the model to an effective form for practical use and demonstrate its adaptability by applying it to layered feedback circuits, resolving long-standing mismatches between model predictions and measured dynamics. These results establish GEAGS as a generalizable platform for predicting emergent behaviors in synthetic gene circuits and underscore the importance of multiscale modeling for robust circuit design in dynamic environments. TeaserMultiscale modeling reveals how growth and proteolysis-linked recycling cause transient oscillations in synthetic gene circuits.

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BibTeXRIS

Namboothiri, H. R., Pandey, A., Hu, C. Y.. 2025-07-09. Resolving Emergent Oscillations in Gene Circuits with a Growth-Coupled Model. https://doi.org/10.1101/2025.07.09.663992

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