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bioRxiv · 10.1101/2025.07.02.662733

Improved Genome Assembly of Onygena corvina, a Keratin-Degrading Fungus

Pavale, S.·Eijsink, V. G. H.·La Rosa, S. L.
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Abstract

ObjectiveOnygena corvina is a non-pathogenic, saprophytic fungus that colonizes feathers, hooves, and hair, and represents a valuable source of keratin-degrading enzymes. Previously, the genome of O. corvina has been assembled based on Illumina short-read sequencing, yielding a reference genome composed of 521 contigs with a contig N50 of 0.229 Mb. ResultsHere, we report an improved O. corvina genome assembly generated using a high-quality hybrid approach that combines Illumina short-read and Oxford Nanopore long-read sequencing. The new assembly consists of only 13 contigs totaling 21.8 Mb, with an N50 of 4.4 Mb, and has a completeness of 98.97%. A total of 7,232 protein-coding genes were identified using an integrative approach that combines de novo predictions, homology-based inferences, and RNA-sequencing-guided evidence. Notably, 158 putative protease-coding genes were identified representing a substantial increase from the 73 predicted proteases in the previous annotation. Our improved genome assembly and associated gene annotations will facilitate comparative genomics, and high-resolution mapping of transcriptomic and proteomic data, to advance research on fungal physiology and fungal abilities to degrade recalcitrant substrates such as keratin.

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Pavale, S., Eijsink, V. G. H., La Rosa, S. L.. 2025-07-03. Improved Genome Assembly of Onygena corvina, a Keratin-Degrading Fungus. https://doi.org/10.1101/2025.07.02.662733

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