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bioRxiv · 10.1101/2025.06.25.661527

A porcine germ cell depletion model to investigate the role of germ cells in gonadal development.

Park, C.-H.·Jeoung, Y.-H.·Yeddula, S. G. R.·Wang, J.·Bhanu, T. P.
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Abstract

The molecular mechanisms regulating the germ cell and gonadal somatic cell development are complex and are still poorly understood in non-rodent mammals. The Nanos family gene-NANOS3 is expressed in the migrating primordial germ cells (PGC) and has key roles in germ cell survival in several species. Herein, we investigated the role of NANOS3 in pigs-an animal model of dual agricultural and biomedical importance. Consistent with prior reports, we found that disruption of NANOS3 expression did not affect PGC specification. Similarly, migration of PGC to the genital ridge occurs normally, but soon followed by an increase in apoptosis and a loss of undifferentiated state by embryonic (e) day 20. Although NANOS3-/- gonads initiate development as ovaries or testes, they displayed structural defects. This is especially pronounced in the ovaries after the sex determination period (e35). Notably, we identified defects in pregranulosa and theca cell development at a single-cell resolution, suggesting that germ cells are important for gonadal somatic cell development. This germ cell depletion model therefore offers novel insights into the potential role of germ cells in the emergence of gonadal somatic lineages.

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Park, C.-H., Jeoung, Y.-H., Yeddula, S. G. R., Wang, J., Bhanu, T. P.. 2025-06-27. A porcine germ cell depletion model to investigate the role of germ cells in gonadal development.. https://doi.org/10.1101/2025.06.25.661527

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