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bioRxiv · 10.1101/2025.06.23.661028

Establishing Vibrio natriegens as a high-performance host for acetate-based poly-3-hydroxybutyrate production

Politan, R. J.·Della Valle, S.·Pineda, L.·Joshi, J.·Euler, C.·Flematti, G.·Fritz, G.
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Abstract

Acetate can be a sustainable and renewable carbon source which holds significant promise for biotechnological production but is underutilized industrially due to limited microbial efficiency. Vibrio natriegens, recognized for exceptionally fast growth rates, represents a compelling host for developing efficient acetate-based bioprocesses. In this study, adaptive laboratory evolution significantly enhanced V. natriegens ability to grow on acetate as the sole carbon source, achieving an 89% increase in growth rate. Genetic and transcriptomic analyses revealed key adaptations improving acetate uptake and metabolism via increased salt tolerance, boosted Pta/AckA pathway activity, and rewired quorum sensing. Further metabolic engineering and bioprocess optimization enabled the evolved strain to reach high cell densities and efficiently convert acetate into the bioplastic poly-3-hydroxybutyrate (PHB), with productivities up to 0.27 g/L/h and PHB accumulation reaching 45.66% of cell biomass. These advances position V. natriegens as a highly promising microbial platform for sustainable, scalable, and cost-effective biomanufacturing using acetate as green feedstock. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=143 SRC="FIGDIR/small/661028v1_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@ebd5b0org.highwire.dtl.DTLVardef@11a28bforg.highwire.dtl.DTLVardef@6950aforg.highwire.dtl.DTLVardef@303d7b_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Politan, R. J., Della Valle, S., Pineda, L., Joshi, J., Euler, C., Flematti, G., Fritz, G.. 2025-06-23. Establishing Vibrio natriegens as a high-performance host for acetate-based poly-3-hydroxybutyrate production. https://doi.org/10.1101/2025.06.23.661028

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