Evolutionary Genomics Guides Scalable Coral Probiotics for Climate Resilience

Xie, M.; Xiang, N.; Liao, T.; Cheung, C. T.; Lee, C. H.; Tse, W. Y.; Tsang, K. K.; Ho, K. E.; He, Q.; Dörr, M.; Manns, H.; Li, P.; Xian, Z.; Wang, X.; Luo, D.; Hayden, R.; Chei, E.; Wan, Z.; Thompson, P.; Brennan, J.; Peixoto, R. S.; Cui, G.; Mcllroy, S. E.; Chui, A. P. Y.; Voolstra, C. R.; Luo, H.

doi:10.1101/2025.06.16.659728

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bioRxiv · 10.1101/2025.06.16.659728

Evolutionary Genomics Guides Scalable Coral Probiotics for Climate Resilience

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Abstract

A universal bottleneck limiting probiotic efficacy in medicine, aquaculture, agriculture, and wildlife conservation is uncertain long-term colonization, necessitating repeated administration. We present an evolution-guided framework for probiotic identification based on genomic hallmarks of emerging host dependency, including widespread pseudogenization and insertion sequence proliferation driving genomic restructuring. Applied to coral reefs, we screened over 1,200 coral-associated bacterial isolates and identified Ruegeria MC10 as exhibiting these signatures. Its presence was associated with increased thermal tolerance of a model cnidarian. Following nursery application, MC10 persisted in reef corals for an 8-month monitoring period through a natural bleaching event, improving color retention and retaining algal photosynthesis performance. This work establishes a predictive, scalable pipeline for selecting persistent probiotics, directly addressing a central constraint on microbiome-based interventions across host systems.

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Xie, M., Xiang, N., Liao, T., Cheung, C. T., Lee, C. H., Tse, W. Y., Tsang, K. K., Ho, K. E., He, Q., Dörr, M., Manns, H., Li, P., Xian, Z., Wang, X., Luo, D., Hayden, R., Chei, E., Wan, Z., Thompson, P., Brennan, J., Peixoto, R. S., Cui, G., Mcllroy, S. E., Chui, A. P. Y., Voolstra, C. R., Luo, H.. 2025-06-16. Evolutionary Genomics Guides Scalable Coral Probiotics for Climate Resilience. https://doi.org/10.1101/2025.06.16.659728

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