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bioRxiv · 10.1101/2025.06.14.659688

Evaporation and Focus Degradation Mitigation in In-Incubator Live Cell Imaging for Capacitance Lab-on-CMOS Microsystem Calibration

Gilpin, Y.·Lin, C.-Y.·Dandin, M. P.
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Abstract

Lab-on-CMOS is an instrumentation technology that combines miniaturized bioanalytical hardware with complementary metal-oxide semiconductor (CMOS) electronics to provide integrated biosensing in a compact format. This paper focuses on a class of lab-on-CMOS systems that utilize capacitance sensing as a means to monitor cell cultures and track cell proliferation, as well as other cell life-cycle events. In this paradigm, changes in interfacial capacitance result from the activity of adherent cells at a bioelectronic interface. These changes are mapped to cell proliferation or life-cycle events using a ground-truth measurement such as live cell imaging from real-time microscopy. This paper identifies instrumentation challenges that arise from conducting these ground-truth measurements in a calibrated cell culture environment, i.e., when the lab-on-CMOS system is deployed inside a CO2 cell culture incubator. We show that autofocusing the microscopy column and provisioning the lab-on-CMOS with an immersion lid are two approaches that significantly improve the quality of live cell imaging ground-truth measurements over long periods.

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Gilpin, Y., Lin, C.-Y., Dandin, M. P.. 2025-06-20. Evaporation and Focus Degradation Mitigation in In-Incubator Live Cell Imaging for Capacitance Lab-on-CMOS Microsystem Calibration. https://doi.org/10.1101/2025.06.14.659688

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