Methanobrevibacter smithii associates with colorectal cancer through trophic control of the cancer bacteriome

Mohammadzadeh, R.; Mahnert, A.; Zurabischvili, T.; Wink, L.; Kumpitsch, C.; Habisch, H.; Sprengel, J.; Filek, K.; Mertelj, P.; Pernitsch, D.; Hingerl, K.; Gorkiewicz, G.; Diener, C.; Loy, A.; Kolb, D.; Trautwein, C.; Madl, T.; Moissl-Eichinger, C.

doi:10.1101/2025.06.12.659283

bioRxiv · 10.1101/2025.06.12.659283

Methanobrevibacter smithii associates with colorectal cancer through trophic control of the cancer bacteriome

Mohammadzadeh, R.·Mahnert, A.·Zurabischvili, T.·Wink, L.·Kumpitsch, C.·Habisch, H.·Sprengel, J.·Filek, K.·Mertelj, P.·Pernitsch, D.·Hingerl, K.·Gorkiewicz, G.·Diener, C.·Loy, A.·Kolb, D.·Trautwein, C.·Madl, T.·Moissl-Eichinger, C.

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Abstract

The human gut is colonized by trillions of microbes that influence the health of their human host. Whereas many bacterial species have now been linked to a variety of different diseases, the involvement of Archaea in human disease remains elusive. Here we searched for gut archaeal signatures of disease by screening 19 cross-sectional clinical studies comprising more than 1,800 individuals. We found that associations between Archaea and medical disorders are common but highly variable and are dominated by a significant increase of Methanobrevibacter smithii in colorectal cancer (CRC) patients. Metabolic modelling and in vitro co-culture identified distinct mutualistic interactions of M. smithii with CRC-causing bacteria such as Fusobacterium nucleatum, including metabolic enhancement. Metabolomics further revealed archaeal-derived compounds with tumor-modulating properties. This provides the first mechanistic link between human gut archaeome and CRC and highlights its role in modulating health in humans through trophic control of the resident bacteriome.

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Mohammadzadeh, R., Mahnert, A., Zurabischvili, T., Wink, L., Kumpitsch, C., Habisch, H., Sprengel, J., Filek, K., Mertelj, P., Pernitsch, D., Hingerl, K., Gorkiewicz, G., Diener, C., Loy, A., Kolb, D., Trautwein, C., Madl, T., Moissl-Eichinger, C.. 2025-06-12. Methanobrevibacter smithii associates with colorectal cancer through trophic control of the cancer bacteriome. https://doi.org/10.1101/2025.06.12.659283

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