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bioRxiv · 10.1101/2025.06.04.657824

Identification of mitochondria and macrophage activation related hub genes in sarcopenia

Wang, Y.·Gu, S.·Li, Y.·Zhou, Y.
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Abstract

BACKGROUNDTo explore hub genes and pathways related to sarcopenia progression associated with mitochondria and macrophage activation. METHODSGEO datasets GSE8479, GSE1428, and GSE136344 were obtained based on GEO database. MRGs and MARGs were acquired. Thereafter, GO, KEGG, GSEA and PPI analysis were implemented. Later, pooled GEO datasets (Combined Datasets) were further obtained to construct the sarcopenia diagnostic model and sarcopenia subtypes. RESULTSA total of 62 M&MARDEGs were finally identified. There were altogether 9 model genes incorporated into the multifactorial regression model. Immunoinfiltration analysis revealed low scores of M&MARDEGs in the samples with sarcopenia. For macrophage activation in sarcopenia samples, most immune cells were strongly correlated in LowScore group (r = -0.707, p < 0.05). Many immune cells in Mitochondria & Macrophage Activation (HighScore) group were strongly correlated with each other (r = -0.661, p < 0.05). CONCLUSIONScreening key genes for sarcopenia associated with mitochondrial and macrophage activation in this study assists in understanding the pathogenesis of sarcopenia and providing new targets for intervention.

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Wang, Y., Gu, S., Li, Y., Zhou, Y.. 2025-06-08. Identification of mitochondria and macrophage activation related hub genes in sarcopenia. https://doi.org/10.1101/2025.06.04.657824

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