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bioRxiv · 10.1101/2025.05.29.656836

Making deep mutational scanning accessible: a cost-efficient approach to construct barcoded libraries for genes of any length

Jann, J.·Gagnon-Arsenault, I.·Pageau, A.·Dube, A. K.·Fijarczyk, A.·Durand, R.·Landry, C. R.
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Abstract

Recent developments in DNA synthesis and sequencing have allowed the construction of comprehensive gene variant libraries and their functional analysis. Achieving high-replication and thorough mutation characterization remains technically and financially challenging for long genes. Here, we developed an efficient, affordable and scalable library construction approach that relies on low-cost DNA synthesis and standard cloning technologies, which will increase accessibility to systematic mutational studies and help advance the field of protein science.

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Jann, J., Gagnon-Arsenault, I., Pageau, A., Dube, A. K., Fijarczyk, A., Durand, R., Landry, C. R.. 2025-05-29. Making deep mutational scanning accessible: a cost-efficient approach to construct barcoded libraries for genes of any length. https://doi.org/10.1101/2025.05.29.656836

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