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bioRxiv · 10.1101/2025.05.23.655761

Nonrandom interchromatin trafficking through dynamic multiphase speckle connections

Abstract

Nuclear speckles (NS) enhance the expression of NS-associated genes, possibly by elevating local levels of factors involved in multiple steps of gene expression. While dozens of large NS are distributed throughout interchromatin regions, the extent to which NS components dynamically redistribute between NS to adjust to local physiological demands remains unknown. Here we used live-cell imaging of endogenous NS proteins to identify an interchromatin network of connections that functionally link NS throughout the nucleus. Over timescales ranging from tens of seconds to minutes, NS material undergoes bulk transfer through these connections. Multiphase NS-connecting structures form through the dynamic juxtapositions of multiple NS component phases. Each phase exhibits distinct yet recurrent viscoelastic dynamics, but together, they integrate into a more stable, multiphase, NS-connecting structure in an ATP-and transcription-dependent manner. Our findings reveal the existence of a cellular mechanism that facilitates coordinated inter-NS protein trafficking through multiphase connections.

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BibTeXRIS

Kim, J., Gonzalez, G. A. H., Venkata, N. C., Han, K. Y., Belmont, A. S.. 2025-05-23. Nonrandom interchromatin trafficking through dynamic multiphase speckle connections. https://doi.org/10.1101/2025.05.23.655761

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