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bioRxiv · 10.1101/2025.05.21.655256

BIO-SPEC: an open-source bench-top parallel bioreactor system.

Parret, L.·Simoens, K.·De Vrieze, J.·Smets, I.
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Abstract

The BIO-SPEC is an open-source, cost-effective, and modular bench-top bioreactor system designed for batch, sequencing batch, and chemostat cultivation. Featuring thermoelectric condensers to eliminate the need for a chiller, it ensures stable long-term operation. Controlled by a Raspberry Pi, the BIO-SPEC offers flexibility in headplate design, gas supply, and feeding strategies, making it a versatile alternative to high-cost commercial systems. This paper details the design, construction, and validation of the BIO-SPEC system, demonstrating its potential to advance microbiology and bioprocessing research through accessible and reliable hardware at a fraction of the cost.

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Parret, L., Simoens, K., De Vrieze, J., Smets, I.. 2025-05-21. BIO-SPEC: an open-source bench-top parallel bioreactor system.. https://doi.org/10.1101/2025.05.21.655256

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