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bioRxiv · 10.1101/2025.05.19.655004

PYEAST - Python Enabled Automated Strain Transformaiton

Madika, A.·Suri, A.·Purohit, A.·Van Raad, D.·Norman, M.·Hartley, C.·Loan, T. D.
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Abstract

Saccharomyces cerevisiae is a widely used biotechnological workhorse in both academic and industrial settings. One reason for its continued popularity is the extensive legacy of genetic tools, developed over its long history of use, that enable precise manipulation of the S. cerevisiae genome. These tools have enabled extensive genetic characterisation and dramatic re-programming efforts for applications ranging from fundamental research to industrial chemical production. Here we present a digital toolkit called PYEAST (Python Enabled Automated Strain Transformation) that encodes some of the most widely used methods for working with S. cerevisiae and modernizes them to leverage advances in DNA synthesis. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=93 SRC="FIGDIR/small/655004v2_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@4584feorg.highwire.dtl.DTLVardef@1e65caorg.highwire.dtl.DTLVardef@1acbe2borg.highwire.dtl.DTLVardef@1f91abc_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Madika, A., Suri, A., Purohit, A., Van Raad, D., Norman, M., Hartley, C., Loan, T. D.. 2025-05-21. PYEAST - Python Enabled Automated Strain Transformaiton. https://doi.org/10.1101/2025.05.19.655004

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