Quercetin treatments alleviate production, egg quality, blood metabolites, and anti-oxidant defence in laying hens : A meta-analysis study

Hartanto, S.; Wardono, H. P.; Kurnianto, H.; Hantoro, F. R. P.; Prasetyo, A.; Haryanto, B.; Hayati, R. N.; Ludfiani, D. D.; Purwasih, R.; Yano, A. A.; Sujiwo, J.; Jang, A.; Sugiharto, S.

doi:10.1101/2025.05.16.654414

bioRxiv · 10.1101/2025.05.16.654414

Quercetin treatments alleviate production, egg quality, blood metabolites, and anti-oxidant defence in laying hens : A meta-analysis study

Hartanto, S.·Wardono, H. P.·Kurnianto, H.·Hantoro, F. R. P.·Prasetyo, A.·Haryanto, B.·Hayati, R. N.·Ludfiani, D. D.·Purwasih, R.·Yano, A. A.·Sujiwo, J.·Jang, A.·Sugiharto, S.

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Abstract

ObjectiveThe study aimed to evaluate impact of quercetin supplementation on performance, egg production, egg quality, blood metabolites, malondialdehyde (MDA) levels and anti-oxidant activity in laying hen MethodsThe meta-analysis study synthesized 27 included studies. We applied mean difference (MD) calculation using restricted maximum likelihood random-effects model to determine the effect size of quercetin treatments. A mixed-effects model was used in subgroup analysis to evaluate the influence of quercetin dose. ResultsQuercetin treatments increased (p<0.05) laying rate (LR) (MD = 2.819%), egg weight (EW) (MD = 1.209 g/unit), haugh unit (HU) (MD = 1.838%), shell thickness (ST) (MD = 0.014 mm), and yolk colour (YC) (MD = 0.526). Feed-to-egg ratio (FER) was reduced (MD = -0.146; p<0.05) in quercetin-treated laying hens. Quercetin decreased (p<0.05) serum glutamic pyruvic transaminase (SGPT) (MD = - 7.009 U/L), glucose (MD = -17.589 mg/dL), and total cholesterol (MD = -20.834) levels in laying hens. High-density lipoprotein (HDL) concentration was improved (MD = 32.590 mg/dL; p<0.05) by quercetin supplementation. Quercetin dietary reduced (MD = -7.373 nmol/mL; p<0.05) MDA level. Moreover, superoxide dismutase (SOD) concentration (MD = 8.114 U/mL; p<0.05) was enhanced in quercetin-administrated laying hens. Quercetin dose linearly affected (p<0.05) the SGPT, glucose, and total cholesterol levels. However, quercetin dose had quadratic effect (p<0.05) on LR, FER, and SOD content. Conclusionquercetin treatments effectively improved performance, egg production, egg quality, blood metabolites, and anti-oxidant defence in laying hens. The effective quercetin dose for optimizing LER, FER, and SOD content was 400 to 600 mg/kg.

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Hartanto, S., Wardono, H. P., Kurnianto, H., Hantoro, F. R. P., Prasetyo, A., Haryanto, B., Hayati, R. N., Ludfiani, D. D., Purwasih, R., Yano, A. A., Sujiwo, J., Jang, A., Sugiharto, S.. 2025-05-21. Quercetin treatments alleviate production, egg quality, blood metabolites, and anti-oxidant defence in laying hens : A meta-analysis study. https://doi.org/10.1101/2025.05.16.654414

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