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bioRxiv · 10.1101/2025.05.01.651609

BRG1 targeting overcomes ABCC-based multidrug resistance induced by paclitaxel

Abstract

Multidrug resistance of cancer cells is attributed to drug-induced alteration of numerous intracellular processes. Using clinically relevant models of triple-negative breast and non-small lung cancer cells we previously showed that these cells respond to repeated paclitaxel exposure by inter alia lysosome enrichment in ABCC3, ABCC5 and ABCC10, which contribute to drug sequestration in these organelles and reduced drug cytotoxicity. In this study we provide experimental evidence that transcription of above mentioned ABCC genes is enabled by BRG1-based SWI/SNF chromatin remodeling complex. Pharmacological inhibition of SWI/SNF with PFI3 or ACBI1, the PROTAC degrader of SMARCA2/4, substantially decline transcription of ABCC3, ABCC5 and ABCC10. Similar effect is caused by transient silencing of SMARCA4 (BRG1), but not SMARCA2 (BRM). The deficiency of BRG1 led to extralysosomal distribution of anticancer drugs, their deeper penetration of spheroids and substantial increase in drug cytotoxicity. Interestingly, in BRG1 deficient cell line paclitaxel triggered mutations, which reverted BRG1 truncating deletion in SMARCA4, thereby restoring SWI/SNF ATPase expression in paclitaxel-resistant cells and increasing transcription of ABCC. Acquisition of drug resistance was associated with BRG1 redistribution in the genome, de novo occurrence at the promoters of genes functionally linked to endo-lysosomal system and stronger co-occurrence with EP300. Our study indicates possible target - SWI/SNF complex for anticancer combinatorial interventions in paclitaxel-induced multidrug resistant phenotypes.

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BibTeXRIS

Gronkowska, K., Michlewska, S., Ploszaj, T., Strachowska, M., Stepien, A., Borowiec, M., Bednarek, A., Robaszkiewicz, A.. 2025-05-07. BRG1 targeting overcomes ABCC-based multidrug resistance induced by paclitaxel. https://doi.org/10.1101/2025.05.01.651609

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