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bioRxiv · 10.1101/2025.04.25.650674

Genomic in vitro transcription and Nanopore direct RNA sequencing of a human B-Lymphocyte cell line

Tzadikario, T.·Akeson, S.·Esfahani, N. G.·Stein, A.·Choudhary, U.·Amar, K.·Jain, M.
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Abstract

Genomic DNA used as a template for in vitro transcription of RNA can serve as a true negative control for benchmarking RNA modification detection by Nanopore direct RNA sequencing (DRS) models. We generated DRS data for in vitro transcribed (IVT) RNA composed of canonical nucleotides using genomic DNA from a human cell line. We applied Dorado modification calling models to these data, and calculated 9-mer specific false-positive rates for eight RNA modifications as a comparison point for future development of RNA modification models. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=119 SRC="FIGDIR/small/650674v2_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@962feorg.highwire.dtl.DTLVardef@4237aborg.highwire.dtl.DTLVardef@154d346org.highwire.dtl.DTLVardef@1fad8f2_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Tzadikario, T., Akeson, S., Esfahani, N. G., Stein, A., Choudhary, U., Amar, K., Jain, M.. 2025-04-27. Genomic in vitro transcription and Nanopore direct RNA sequencing of a human B-Lymphocyte cell line. https://doi.org/10.1101/2025.04.25.650674

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