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bioRxiv · 10.1101/2025.04.11.648033

Light-Controlled Synthetic Communication Networks via Paired Connexon Nanopores

Abstract

Living cells employ dynamic networks for intercellular communication and cooperation, leading to tissue-wide activity. One emerging challenge in the field of bottom-up synthetic biology is emulating such sophisticated behaviors in liposome-based synthetic cells (SCs). Fabricating communication networks in lipid bilayer-based SCs remains a challenge as signaling molecules must transit through two consecutive membranes to transfer information between different SCs. Here, we address this obstacle by engineering connexin channels that directly connect the lumens of adhering SC membranes. We focus on orthogonal channel-forming connexins, namely connexin 43 and connexin 32, and re-design their channel activity to be UV- and near IR-responsive, respectively. By combining engineered connexins into a single SC assembly, we demonstrate orthogonal transfer of reactive signaling molecules between SCs, giving rise to unique reaction products and network states in a wavelength-dependent manner - an important step toward synthetic communication networks.

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BibTeXRIS

Sihorwala, A. Z., Lin, A. J., Ramirez-Velez, I., Yang, S., Chowdhury, S., Stachowiak, J. C., Belardi, B.. 2025-04-11. Light-Controlled Synthetic Communication Networks via Paired Connexon Nanopores. https://doi.org/10.1101/2025.04.11.648033

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