The ribosome directs nascent chains through two folding-dependent pathways

Mitropoulou, A. N.; Wlodarski, T. N.; Streit, J. O.; Chan, S. H. S.; Plessa, E.; Woodburn, L. F.; Cabrita, L. D.; Christodoulou, J.

doi:10.1101/2025.04.08.647855

bioRxiv · 10.1101/2025.04.08.647855

The ribosome directs nascent chains through two folding-dependent pathways

Mitropoulou, A. N.·Wlodarski, T. N.·Streit, J. O.·Chan, S. H. S.·Plessa, E.·Woodburn, L. F.·Cabrita, L. D.·Christodoulou, J.

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Abstract

During their vectorial biosynthesis on the ribosome, elongating nascent polypeptide chains explore a range of conformational states towards their biologically functional structure. However, this high structural heterogeneity has limited their observation at high-resolution. Here, we have used an integrated structural biology approach to explore the structures of the multi-domain immunoglobulin-like FLN5-6 during its biosynthesis, capturing early folding through to native folding. We developed an in-silico purification approach for cryo-EM of ribosome-nascent chain complexes (RNCs), and integrated the resulting cryo-EM maps with NMR spectroscopy and atomistic molecular dynamics (MD) simulations to produce experimentally reweighted structural ensembles of RNC. The resulting atomistic structures reveal insights into the orientational heterogeneity of the nascent chain and its dynamic interactions with the ribosome. In particular, we find that two distinct pathways exist for nascent polypeptides in the exit tunnel vestibule, influenced by their stage of biosynthesis, folding conformational state and ribosomal RNA helices lining the tunnel. Our systematic analysis of the structures of nascent proteins translation-stalled at multiple time-points provides insights into how the ribosome dynamically modulates its pathway out of the exit tunnel to regulate its folding and accessibility for auxiliary factors of other co-translational events.

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Mitropoulou, A. N., Wlodarski, T. N., Streit, J. O., Chan, S. H. S., Plessa, E., Woodburn, L. F., Cabrita, L. D., Christodoulou, J.. 2025-04-10. The ribosome directs nascent chains through two folding-dependent pathways. https://doi.org/10.1101/2025.04.08.647855

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