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bioRxiv · 10.1101/2025.04.03.647078

Rheinheimera palamurensis sp. a novel bacterium isolated from drinking water resources

Paul, T. R.·Shiva Shanker, A.·Kumar, D. K. S.·Srinivasulu, D.·Vootla, P. K.·Pindi, P. K.
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Abstract

A bacterial strain designated NG3T was isolated from a drinking water samples collected from Nagar Kurnool, Telangana State, India (16{degrees} 29 14.5356 N ; 78{degrees} 18 33.966) and characterized using a polyphasic taxonomic approach, Strain NG3T was Gram-negative, aerobic, non pigmented and motile by means of a single polar flagellum. Growth occurred at 18 - 37{degrees} C (optimum 27 - 32{degrees} C), pH at 6.5 - 8.0 (Optimum 7.0 - 7.5) and in the presence of 0-2% NaCl (Optimum 0.7-1.0%), Phylogenetic analyses based on 16S rRNA gene sequence showed that strain NG3T belonged to the genus Rheincheimera and its most closely related neighbor was Rheinheimera aquatica GR5T (GQ168584) with sequence similarity of 99 %. The cellular fatty acid composition of strain NG3T showed a spectrum of 11 fatty acids with a pronounced dominance of saturated fatty acids (82.7 %), including a high abundance of C16 : 0, C18 : 1{omega}7c, and C12 : 0 3-OH. The major respiratory quinone was MK-7 and Q-8. The DNA G+C content of the genomic DNA was 48.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, aminolipid and two uncharacterized phospholipids. The DNA-DNA relatedness of strain NG3T with respect to recognized members of the genus Rheinheimera was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NG3T represents a novel species in the genus Rheinheimera, for which the name Rheinheimera palamurensis sp. nov. was proposed. The type strain is NG3T (=GU566360=JCM16716=KCTC23111).

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Paul, T. R., Shiva Shanker, A., Kumar, D. K. S., Srinivasulu, D., Vootla, P. K., Pindi, P. K.. 2025-04-04. Rheinheimera palamurensis sp. a novel bacterium isolated from drinking water resources. https://doi.org/10.1101/2025.04.03.647078

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