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bioRxiv · 10.1101/2025.04.01.646544

A non-vesicular Argonaute protein is transmitted from nematode to mouse and is important for parasite survival

Abstract

Argonautes are ancient proteins with well-characterised functions in cell-autonomous gene regulation and genome defense but less clear roles in non-cell-autonomous processes. Extracellular Argonautes have been reported across plants, animals and protozoa yet their biochemical and functional properties remain elusive. Here we demonstrate that an extracellular Argonaute (exWAGO) released by the rodent-infective parasitic nematode Heligmosomoides bakeri is detectable inside mouse cells during the natural infection. We further show that exWAGO is released from H.bakeri in both vesicular and non-vesicular forms that have different resistances to proteolysis, different accessibilities to antibodies and associate with different subsets of secondary siRNAs. Using recombinant exWAGO protein we demonstrate that non-vesicular exWAGO is directly internalised by mouse cells in vitro and that immunisation of mice with exWAGO confers partial protection against subsequent H. bakeri infection and generates antibodies that block exWAGO uptake into cells. Finally, we show that properties of exWAGO are conserved across Clade V nematodes that infect humans and livestock. Together this work expands the context in which Argonautes function and illuminates an RNA-binding protein as a vaccine target for parasitic nematodes.

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BibTeXRIS

Neophytou, K., Martinez-Ugalde, I., Fenton, T., Robertson, E., Strachan, L. J., Harcus, Y., Naar, C. M., Wright, D., Price, D. R. G., White, R., Evans, M. J., Bermudez-Barrientos, J. R., Li, H., Maizels, R. M., Aroian, R. V., Nisbet, A. J., Abreu-Goodger, C., Buck, A. H.. 2025-04-01. A non-vesicular Argonaute protein is transmitted from nematode to mouse and is important for parasite survival. https://doi.org/10.1101/2025.04.01.646544

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