Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2025.03.31.646393

Iron acquisition in the weevil-farming fungus Penicillium herquei: Implications of mineral elements in insect-fungus mutualistic association

Qiu, P.·Liu, X.·Wei, D.
View original source ↗Export metadata ↓

Abstract

Mutualistic interactions between insects and fungi are pivotal in ecosystem dynamics, yet the underlying molecular mechanisms remain largely unexplored. This study investigates iron acquisition strategies of the weevil-farming Penicillium herquei, revealing the involvement of mineral elements in insect-fungus symbiosis. Comparative transcriptomics of weevil-farming strain (WFS) and soil free-living strain (SFS) revealed distinct transcriptional profiles, with 4,357 up-regulated genes in WFS. Enrichment analyses highlighted a significant up-regulation of genes linked to oxidoreductase activity, iron and heme binding, with a notable prevalence of Cytochrome P450 (CYP450). qRT-PCR confirmed differential expression of CYP450 and siderophore-related genes, indicating enhanced iron absorption in WFS. Comparative analysis of iron content further demonstrated significantly higher iron levels in WFS than in SFS and weevil host plant leaves, suggesting a nutritional adaptation for symbiotic lifestyle. These findings provide novel insights into the role of iron metabolism in insect-fungus mutualism, highlighting potential evolutionary mechanisms that bolster symbiotic fitness. IMPORTANCEUnraveling the complex interplay between insects and fungi is crucial for deciphering the intricate dynamics of ecosystems. In this study a notable up-regulation of genes associated with iron and heme binding, as well as a significant increase in iron content within WFS, suggesting a specialized adaptation for enhanced iron acquisition, potentially enabling the fungus to efficiently provide essential nutrients, including bioavailable iron, to weevil host. This research not only advances our understanding of the molecular mechanisms governing insect-fungus mutualism but also highlights the potential evolutionary mechanisms that bolster symbiotic fitness and contribute to the co-evolution of these interacting species.

Explore related subjects

microbiologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Qiu, P., Liu, X., Wei, D.. 2025-04-01. Iron acquisition in the weevil-farming fungus Penicillium herquei: Implications of mineral elements in insect-fungus mutualistic association. https://doi.org/10.1101/2025.03.31.646393

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

A population-scale landscape of the subgingival microbiome reveals divergent routes to periodontal dysbiosis

Periodontitis is an archetypical mucosal inflammatory disease in which microbiome dysbiosis at the tooth-epithelial interface interacts with host genetic and behavioral risk factors to drive immune-mediated tissue destruction. Although subgingival microbiome compositional shifts are thought to parallel disease severity, microbiome variation at the population-level and its relationship to periodontal clinical phenotypes and disease-modifying factors remain poorly defined. Here, we use unsupervised manifold learning to map the compositional landscape of the subgingival microbiome in 1,355 adults spanning periodontal health to severe periodontitis. We identified eight latent microbiome states organized along a branching continuum from eubiosis to dysbiosis. An intermediate microbial configuration marked ecological destabilization and bifurcation into two distinct periodontitis-associated dysbiotic trajectories, distinguished by links to gingival inflammation and smoking. Although the microbiome trajectories broadly tracked periodontal destruction, a minority of individuals showed discordant microbiome-clinical phenotypes, with some individuals with periodontitis retaining otherwise eubiotic microbiomes enriched for low-abundance pathobionts, while some cases of health or mild disease had highly dysbiotic communities, suggesting distinct host susceptibility. Together, these findings define a population-scale ecological landscape of the subgingival microbiome, reveal divergent trajectories to periodontal dysbiosis, and highlight heterogeneity in the relationship between microbial community structure and clinical disease expression.

microbiology↗
bioRxiv2026

The iron-binding siderophore enterobactin is required for the response of multi-drug resistant Klebsiella pneumoniae to zinc limitation

To persist during infection Klebsiella pneumoniae must overcome nutrient iron and zinc limitation imposed by the host immune system through a process called nutritional immunity. Secreted small molecule siderophores are a major virulence determinant of Klebsiella pneumoniae pathogenesis and are presumed to overcome nutritional immunity by binding iron for bacterial acquisition. In this work, we set out to identify how a multi-drug resistant K. pneumoniae grows in zinc limited environments. Using unbiased transcriptomics, proteomics, and an arrayed transposon screen, we identified that synthesis and uptake of the siderophore enterobactin is required to allow for growth in low zinc conditions. Iron-specific chelators did not replicate this phenotype and addition of supplemental iron through heme in growth media could not complement severe growth defects of enterobactin mutant K. pneumoniae experiencing zinc limitation. Finally, zinc starvation induced enterobactin production independent of the canonical zinc uptake regulator (Zur) transcription factor suggesting an unidentified regulatory mechanism by which Gram-negative pathogens may respond to zinc stress. Together, these studies expand the role of enterobactin beyond iron regulation and highlight a previously unreported link between iron and zinc homeostasis in Klebsiella pneumoniae.

microbiology↗
bioRxiv2026

A microbiota-derived protease links phage susceptibility to host epithelial responses

Bacteriophages are major ecological drivers of gut microbial ecology, yet whether bacterial mechanisms that determine phage susceptibility have consequences for the mammalian host remains poorly understood. Here, we identify dipeptidyl peptidase 11 (Dpp11a), the predominant active serine protease of the prevalent gut commensal Phocaeicola vulgatus, as an unexpected bacterial defence factor. Dpp11a protects against environmental proteases and confers resistance to bacteriophage infection. Metatranscriptomic analyses further reveal increased expression of both dpp11a and P. vulgatus-associated phage transcripts in ulcerative colitis stool samples, indicating that both components of this interaction are transcriptionally active in disease-associated human microbiomes. Using the microfluidic gut-on-a-chip co-culture model HuMiX, we show that the absence of Dpp11 is accompanied by altered epithelial tight-junction remodelling during phage-bacterial infection. Together, our findings reveal that the consequences of bacterial phage defence can extend beyond phage-bacterium interactions to the mammalian epithelium.

microbiology↗