A subset of dorsal raphe dopamine neurons is critical for survival-oriented vigilance.

Guillaumin, A.; Dhellemmes, T.; Perrot, E.; Boi, L.; De Castro Medeiros, D.; Glangetas, C.; Dumas, S.; Dovero, S.; Biendon, N.; Ladeveze, E.; Landry, M.; Bezard, E.; Baufreton, J.; Fisone, G.; Georges, F.

doi:10.1101/2025.03.31.646345

bioRxiv · 10.1101/2025.03.31.646345

A subset of dorsal raphe dopamine neurons is critical for survival-oriented vigilance.

Guillaumin, A.·Dhellemmes, T.·Perrot, E.·Boi, L.·De Castro Medeiros, D.·Glangetas, C.·Dumas, S.·Dovero, S.·Biendon, N.·Ladeveze, E.·Landry, M.·Bezard, E.·Baufreton, J.·Fisone, G.·Georges, F.

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Abstract

Defensive behaviors are essential for survival, with risk assessment enabling organisms to evaluate and respond to potential threats. The dorsal raphe nucleus (DRN), a key neuromodulatory center, is crucial for encoding motivational salience and regulating arousal and sleep-wake states through its diverse neuronal populations, including dopaminergic neurons (DRNDA). While the roles of DRNDA neurons have been studied, their specific contributions to threat evaluation are less understood. Recent research identifies a distinct subset of DRNDA neurons that express vasoactive intestinal peptide (VIP) and project to the central amygdala (CeA) and the oval nucleus of the bed nucleus of the stria terminalis (ovBNST). Together, these two regions comprise the central extended amygdala, a key network regulating adaptive responses to threats. We hypothesized that distinct DRNDA subpopulations exert diverging effects on sleep-wake regulation and that DRNVIP neurons play a pivotal role in coordinating activity between the CeA and ovBNST, thereby influencing risk assessment and defensive response. To test this hypothesis, we used a combination of in situ hybridization, immunochemistry, whole-brain mapping, electrophysiology, and cell-specific genetic tools in mice and non-human primates. Our findings reveal that DRNVIP neurons form a key DRNDA neuronal subset, uniquely positioned to regulate the central extended amygdala through a feedback loop. These neurons receive inputs from Protein Kinase C delta (PKC-{delta}) neurons in the ovBNST and CeA and send glutamate-releasing projections back to these regions, modulating PKC-{delta} neuron excitability. Selective ablation of DRNVIP neurons increases activity in both the BNST and CeA, disrupting active-phase sleep architecture and impairing risk assessment and defensive behaviors. Together, these findings suggest DRNVIP neurons control specific phases of sleep and orchestrate the central extended amygdalas role in risk assessment and defensive responses. HIGHLIGHTSO_LIDRNVIP neurons form a subset of DRNDA neurons in mice and non-human primates. C_LIO_LIDRNVIP receive inputs from Protein Kinase C delta (PKC-{delta}) neurons in the ovBNST and CeA and project back to both. C_LIO_LIBy releasing glutamate, DRNVIP neurons regulate PKC-{delta} neuron excitability in the ovBNST and CeA. C_LIO_LIAblating DRNVIP neurons increases BNST and CeA activity, disrupts active-phase sleep architecture, and impairs threat responses. C_LI IN BRIEFDRNVIP neurons, a key subset of DRNDA neurons in mice and primates, are strategically positioned to influence the central extended amygdala via feedback loops. They regulate PKC-{delta} neuron excitability in the ovBNST and CeA through glutamate release, with their ablation heightening activity in these regions and altering active-phase sleep architecture, risk assessment and defensive behaviors.

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Guillaumin, A., Dhellemmes, T., Perrot, E., Boi, L., De Castro Medeiros, D., Glangetas, C., Dumas, S., Dovero, S., Biendon, N., Ladeveze, E., Landry, M., Bezard, E., Baufreton, J., Fisone, G., Georges, F.. 2025-04-01. A subset of dorsal raphe dopamine neurons is critical for survival-oriented vigilance.. https://doi.org/10.1101/2025.03.31.646345

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