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bioRxiv · 10.1101/2025.03.24.645130

Structural decay of poly(ethylene terephthalate) by enzymatic degradation

Tadokoro, D.·Imai, T.
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Abstract

Synthetic polymers, such as plastics, are ubiquitous materials that are used in many applications. The sustainable use of plastics is becoming increasingly important given the emergent issues of environmental pollution by microplastics and the limited resources of petroleum on Earth. One of the key strategies for the sustainable use of plastics is recycling. Enzymatic degradation is a promising technique for recycling plastic because this process requires neither energy nor harsh solvents, such as strong alkaline solutions and organic solvents. In this study, the enzymatic degradation of poly(ethylene terephthalate) (PET), a major plastic used in daily life, was investigated, aiming to improve the efficiency of enzymatic degradation by understanding the decay of the polymeric PET structure. The structural decay of an amorphous PET film induced by a PET-hydrolyzing enzyme (PETase) was analyzed using wide-angle X-ray diffraction (WAXD), small-angle X-ray scattering (SAXS), electron microscopy, and X-ray computed tomography (X-ray CT). Structural decay progressed from the surface of the film, and many nested pores (10-8-10-5 m) were found in the later stage of degradation, reflecting the structural difference between the surface and core of the material. Information regarding the polymeric structure of the plastic under enzymatic degradation is important for improving the degradation performance of enzymes for realizing the biochemical recycling of plastics such as PET. HighlightsO_LIPETase-induced decay of a PET film was analyzed regarding its polymer structure C_LIO_LIDecay occurred from the surface to form multiscale pores of 10-5 to 10-8 m C_LIO_LIThe PET decay mechanism differs from that of cellulose, a typical biopolymer C_LIO_LICellulose biodegradation is a good example to learn biodegradable PET materials C_LI

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Tadokoro, D., Imai, T.. 2025-03-27. Structural decay of poly(ethylene terephthalate) by enzymatic degradation. https://doi.org/10.1101/2025.03.24.645130

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