In vitro approaches to study centriole and cilium function in early mouse embryogenesis

Abstract

Although centrioles and primary cilia play an essential role in early mammalian development, their specific function during the interval between their initial formation and the subsequent arrest of embryogenesis in embryos deficient in centrioles or cilia remains largely unexplored. Here, we demonstrate that different 3D in vitro model systems recapitulate early centriole and cilium formation in mouse development. Centrioles and cilia are dispensable in 3D in vitro mouse rosettes, a model system that mimics key events of implantation, including polarization and lumenogenesis. In gastruloids, a model system that recapitulates developmental processes up to 8.5 days after fertilization, centriole loss results in early disassembly. In contrast, cells devoid of cilia continue to form elongated, differentiated and polarized gastruloids, with minor differences at 96 h. Finally, we show that in a mutant affecting the centriolar distal appendages, cilia are absent from 2D cultures but are capable of forming in 3D rosettes and gastruloids, highlighting the importance of multifactorial 3D environment setups in developmental studies. SummaryThis study presents the first in vitro analysis of centriole and cilium formation during early mouse embryonic development, using 3D models to mimic implantation, tissue patterning, and axis elongation, offering a controlled platform for investigating their roles in embryogenesis.