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bioRxiv · 10.1101/2025.03.06.641745

DNA-PKcs governs LAT-dependent signaling in CD4+ and CD8+ T cells

Abstract

Formation of the immune synapse (IS) following T cell antigen recognition includes recruitment of the Linker for Activation of T cells (LAT). Once at the IS, LAT tyrosines are phosphorylated allowing it to serve as a scaffold for formation of the "signalosome", a multiprotein complex that drives TCR signaling. Here, we show that upon T cell activation, DNA dependent protein kinase catalytic subunit (DNA-PKcs) interacts with LAT and localizes to the IS. Inhibition of DNA-PKcs diminishes LAT localization at the IS. We identified two LAT serines phosphorylated by DNA-PKcs, S224 and S241, that impact LAT tyrosine phosphorylation, protein binding, and cytokine production. Using our mouse model designed to delete DNA-PKcs expression within mature CD4+ or CD8+ T cells, we show loss of DNA-PKcs results in T cells unable to control tumor growth or induce allogeneic graft rejection. These data bring to the forefront DNA-PKcs as a pivotal protein in T cell function.

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Rainwater, R. R., Azevedo-Pouly, A., Waldrip, Z., Hicks, B. H., Callais, N. A., Koss, B., Storey, A. J., Burdine, L., Burdine, M.. 2025-03-10. DNA-PKcs governs LAT-dependent signaling in CD4+ and CD8+ T cells. https://doi.org/10.1101/2025.03.06.641745

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