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bioRxiv · 10.1101/2025.03.06.641285

A patient-derived ovarian cancer organoid platform to study susceptibility to natural killer cells

Mercadante, M.·Scheben, A.·Estrada, J.·Savas-Carstens, J.·Sullivan, W.·Housel, N.·Volpari, T.·Hebner, J.·Sapar, M.·Rusielewicz, T.·Monsma, F. J.·Semrau, S.·Wang, Y.·Martin, L. A.
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Abstract

Intratumoral heterogeneity drives therapy resistance and relapses in advanced stage cancers, such as ovarian cancer. Here, we present a live cell imaging assay using patient-derived ovarian cancer organoids for real time capture and quantification of natural killer cell-mediated apoptotic events in >500 organoids simultaneously. Our assay revealed significant inter- and intratumor response heterogeneity and identified a rare resistant organoid population, opening avenues to test immunomodulatory strategies that overcome resistance.

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Mercadante, M., Scheben, A., Estrada, J., Savas-Carstens, J., Sullivan, W., Housel, N., Volpari, T., Hebner, J., Sapar, M., Rusielewicz, T., Monsma, F. J., Semrau, S., Wang, Y., Martin, L. A.. 2025-03-07. A patient-derived ovarian cancer organoid platform to study susceptibility to natural killer cells. https://doi.org/10.1101/2025.03.06.641285

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