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bioRxiv · 10.1101/2025.03.02.640994

Programming cellular condensates for living materials using an intrinsically disordered protein display platform

Chang, R.·Tu, H.·Joshi, N. S.
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Abstract

Self-organization of multicellular systems is vital for building structure in living system but remains underexplored in engineered living materials. We developed iDP2, a platform enabling high-density display of intrinsically disordered proteins on the surface of E. coli using CsgF as a surface-bound anchor protein to which other protein domains can be fused. Display depends on a lack of intrinsic structure and enable multivalent weak interactions that drive environmental responsiveness and sequence-programmable self-segregation. iDP2 allow the programming of material properties like viscoelasticity, stability, offering a method to rationally control cell-cell interactions. This approach could be applied to engineer self-organizing tissues or materials with customized properties.

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Chang, R., Tu, H., Joshi, N. S.. 2025-03-03. Programming cellular condensates for living materials using an intrinsically disordered protein display platform. https://doi.org/10.1101/2025.03.02.640994

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