Ethylene modulates cell wall mechanics for root responses to compaction

Zhang, J.; Qu, Z.; Liu, Z.; Li, J.; Farrar, E.; Chara, O.; Ogorek, L. P.; Borges, A.; Sakamoto, S.; Mitsuda, N.; Zhu, X.; Zhu, M.; Shi, J.; Liang, W.; Bennett, M.; Pandey, B.; Zhang, D.; Persson, S.

doi:10.1101/2025.03.02.640043

bioRxiv · 10.1101/2025.03.02.640043

Ethylene modulates cell wall mechanics for root responses to compaction

Zhang, J.·Qu, Z.·Liu, Z.·Li, J.·Farrar, E.·Chara, O.·Ogorek, L. P.·Borges, A.·Sakamoto, S.·Mitsuda, N.·Zhu, X.·Zhu, M.·Shi, J.·Liang, W.·Bennett, M.·Pandey, B.·Zhang, D.·Persson, S.

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Abstract

Soil stresses impact crop yields, presenting global agricultural challenges. Soil compaction triggers root length reduction and radial expansion driven by the plant hormone ethylene. We report how ethylene controls cell wall properties to promote root radial expansion. We demonstrate how soil compaction stress, via ethylene, upregulates Auxin Response Factor1 in the root cortex, which represses Cellulose Synthase (CESA) genes. CESA repression drives radial expansion of root cortical cells by modifying the thickness and mechanics of their cell walls, which result in a "stiff epidermis-soft cortex" contrast. Our research thus connects ethylene signaling with root mechanics via cell wall strength, and reveals how dynamic regulation of cellulose synthesis crucially controls root growth in compacted soil.

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Zhang, J., Qu, Z., Liu, Z., Li, J., Farrar, E., Chara, O., Ogorek, L. P., Borges, A., Sakamoto, S., Mitsuda, N., Zhu, X., Zhu, M., Shi, J., Liang, W., Bennett, M., Pandey, B., Zhang, D., Persson, S.. 2025-03-03. Ethylene modulates cell wall mechanics for root responses to compaction. https://doi.org/10.1101/2025.03.02.640043

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