TMEM145 is a key component in stereociliary link structures of outer hair cells

Roh, J. W.; Oh, K. S.; Lee, J.; Choi, Y.; Kim, S.; Hong, J. W.; Kim, Y.; Lew, H.; Jang, S. H.; Shin, H.-S.; Ohk, J.; Jung, H.; Seo, K. Y.; Bok, J.; Kim, C. H.; Gee, H. Y.

doi:10.1101/2025.02.10.637577

bioRxiv · 10.1101/2025.02.10.637577

TMEM145 is a key component in stereociliary link structures of outer hair cells

Roh, J. W.·Oh, K. S.·Lee, J.·Choi, Y.·Kim, S.·Hong, J. W.·Kim, Y.·Lew, H.·Jang, S. H.·Shin, H.-S.·Ohk, J.·Jung, H.·Seo, K. Y.·Bok, J.·Kim, C. H.·Gee, H. Y.

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Abstract

Outer hair cells (OHCs) in the cochlea contain specialized stereociliary structures essential for auditory function. These include horizontal top connectors (HTCs), linking adjacent stereocilia and tectorial membrane-attachment crowns (TM-ACs), anchoring the tallest stereocilia to the tectorial membrane. The known molecular components of these structures, such as stereocilin, otogelin, otogelin-like, and tubby, lack transmembrane domains, suggesting the existence of anchoring proteins. This study identified TMEM145, a transmembrane protein with a Golgi dynamics (GOLD) domain, as a crucial OHC stereocilia component. TMEM145 was expressed in both OHCs and spiral ganglion neurons, with specific localization to TM-ACs and HTCs in OHCs. Tmem145 knockout (KO) mice exhibited profound hearing impairment at three weeks of age, with complete loss of distortion product otoacoustic emissions, indicating OHC dysfunction. Immunostaining and scanning electron microscopy revealed the absence of TM-ACs and HTCs in Tmem145 KO mice. In heterologous cell systems, TMEM145 interacted with stereocilin and tubby, facilitating their extracellular secretion. TMEM145 was undetectable in stereocilin KO and tubby mutant mice, indicating interdependence among these proteins. These findings establish TMEM145 as an essential membrane protein for the structural integrity of OHC stereocilia, providing insights into the molecular architecture of cochlear hair cells and their role in auditory function.

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Roh, J. W., Oh, K. S., Lee, J., Choi, Y., Kim, S., Hong, J. W., Kim, Y., Lew, H., Jang, S. H., Shin, H.-S., Ohk, J., Jung, H., Seo, K. Y., Bok, J., Kim, C. H., Gee, H. Y.. 2025-02-12. TMEM145 is a key component in stereociliary link structures of outer hair cells. https://doi.org/10.1101/2025.02.10.637577

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