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bioRxiv · 10.1101/2025.02.05.635995

Loss of intestinal endosome associated protein sorting nexin 27 disrupts epithelial barrier and promotes inflammation

Deb, S.·Zhang, Y.-g.·Xia, Y.·Sun, J.
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Abstract

Backgrounds and aimsSNX27, member of the sorting nexin (SNX) family, carries a unique PDZ domain and mediates recycling of endocytosed transmembrane proteins. SNX27 is critical for neurodevelopmental processes, however its role in intestine remains unexplored. We aim to determine the previously unknown roles of SNX27 in regulating intestinal homeostasis, epithelial barrier integrity, and inflammatory responses. MethodsWe used available datasets to analyze SNX27 expression in human IBD. We generated a novel mouse model of SNX27 conditional deletion from intestinal epithelial cells (SNX27{Delta}IEC) and challenged these mice with Dextran Sulfate Sodium (DSS). ResultsSNX27 expression was significantly lower in human IBD, including UC and CD. SNX27{Delta}IEC mice had significantly lower bodyweight and exhibited increased proliferation and poor differentiation of secretory Paneth and Goblet cells. We found reduced mucin layer and downregulation of crucial epithelial barrier proteins {Delta}-catenin, E-cadherin, ZO-1, and Claudin10 in SNX27{Delta}IEC mice. SNX27{Delta}IEC mice showed high intestinal permeability and spontaneously developed intestinal inflammation. Moreover, SNX27{Delta}IEC mice were more susceptible towards DSS-induced colitis, compared to the SNX27Loxp mice. ConclusionOverall, deletion of intestinal epithelial SNX27 weakens barrier functions and promotes inflammation. Our results indicate a novel role of SNX27 in regulating intestinal physiology and protecting against intestinal disorders. Thus, understanding the mechanisms of SNX27 downregulation in IBD will provide insights into new prevention and targets against chronic inflammation. SynopsisO_LISNX27 recycles internalized transmembrane proteins in the endocytic pathway. Human IBD showed reduced levels of SNX27. C_LIO_LISNX27 plays novel functions by maintaining intestinal homeostasis and inhibiting inflammation. C_LIO_LISNX27 protects the host against losing intestinal integrity during inflammation. C_LI

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Deb, S., Zhang, Y.-g., Xia, Y., Sun, J.. 2025-02-07. Loss of intestinal endosome associated protein sorting nexin 27 disrupts epithelial barrier and promotes inflammation. https://doi.org/10.1101/2025.02.05.635995

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