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bioRxiv · 10.1101/2025.02.01.636078

Dose-dependent changes in global brain activity and functional connectivity following exposure to psilocybin: a BOLD MRI study in awake rats.

Fuini, E.·Chang, A.·Ortiz, R.·Nasseef, T.·Edwards, J.·Latta, M.·Gonzales, E.·Woodward, T.·Bradshaw, H.·Axe, B.·Maheswari, A.·Cavallaro, N.·Kulkarni, P.·Ferris, C.
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Abstract

Psilocybin is a hallucinogen with complex neurobiological and behavioral effects. This is the first study to use MRI to follow functional changes in brain activity in response to different doses of psilocybin in fully awake, drug naive rats. Female and male rats were given IP injections of vehicle or psilocybin in doses of 0.03 mg/kg, 0.3 mg/kg, and 3.0 mg/kg while fully awake during the imaging session. Changes in BOLD signal were recorded over a 20 min window. Data for resting state functional connectivity were collected approximately 35 min post injection All data were registered to rat 3D MRI atlas with 173 brain regions providing site-specific changes in global brain activity and changes in functional connectivity. Treatment with psilocybin resulted in a significant dose-dependent increase in positive BOLD signal. The areas most affected by the acute presentation of psilocybin were the somatosensory cortex, basal ganglia and thalamus. Females were significantly more sensitive to the 0.3 mg/kg dose of psilocybin than males. There was a significant dose-dependent global increase in functional connectivity, highlighted by hyperconnectivity to the cerebellum. Brain areas hypothesized to be involved in loss of sensory filtering and organization of sensory motor stimuli such as the claustrum and the cortico-basal ganglia-thalamic-cortical loop were all affected by psilocybin in a dose-dependent manner. Indeed, the general neuroanatomical circuitry associated with the psychedelic experience was affected but the direction of the BOLD signal and pattern of activity between neural networks was inconsistent with the human literature.

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Fuini, E., Chang, A., Ortiz, R., Nasseef, T., Edwards, J., Latta, M., Gonzales, E., Woodward, T., Bradshaw, H., Axe, B., Maheswari, A., Cavallaro, N., Kulkarni, P., Ferris, C.. 2025-02-06. Dose-dependent changes in global brain activity and functional connectivity following exposure to psilocybin: a BOLD MRI study in awake rats.. https://doi.org/10.1101/2025.02.01.636078

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