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bioRxiv · 10.1101/2025.01.31.635854

Structural and Functional Versatility of the Amyloidogenic Antimicrobial Peptide Citropin 1.3

Abstract

Citropin 1.3 is an antimicrobial peptide produced by the amphibian Litoria citropa (Southern bell frog), which self-aggregates into distinct fibrillar structures, however, the function of the fibrils remains unclear and largely unexplored. In this study, the structural and functional properties of citropin 1.3 were investigated using cryogenic electron microscopy and fluorescence microscopy in the presence of membrane and cell models, and with X-ray crystallography. Canonical amyloids, multilayered nanotubes, and a novel mixed fibril were observed. Experiments with negatively charged giant unilamellar vesicles revealed that the peptide facilitates membrane fusion while simultaneously undergoing phase separation in the presence of phospholipids. In presence of mammalian cells, citropin 1.3 permeabilizes membranes, leading to cell death, and over time, colocalizes with genetic material. Overall, this work provides new insights into the structural dynamics of the amyloidogenic antimicrobial peptide citropin 1.3 and its interactions with different systems.

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BibTeXRIS

Strati, F., Pigozzi Cali, M., Bloch, Y., Mostafavi, S., Monistrol, J., Golubev, A., Rayan, B., Gustavsson, E., Landau, M.. 2025-01-31. Structural and Functional Versatility of the Amyloidogenic Antimicrobial Peptide Citropin 1.3. https://doi.org/10.1101/2025.01.31.635854

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