Fermentative yeast diversity at the northern range limit of their oak tree hosts

Abstract

Fermentative yeasts play important roles in both ecological and industrial processes but their distribution and abundance in natural environments is not well understood. We investigated the diversity of yeast species associated with oak trees (Quercus spp.) in the northern range limit of oak in Sweden, and identified climatic and ecological conditions governing their distribution. We isolated yeasts from oak bark collected from 28 forests and identified them to the species level using metabarcoding. Most communities were dominated by species in the Saccharomycetaceae family, especially by species of Saccharomyces, Kluyveromyces, and Pichia. Each of these genera showed a distinct latitudinal and longitudinal distribution, and both temperature and precipitation metrics predicted significant variation in their abundance. Consistent with this, laboratory assays revealed significant effects of temperature on the growth of strains collected from different longitudes and latitudes. We further found that older trees harbour more diverse and more balanced microbial communities with more evenly distributed species abundances, and that communities across trees were more similar when sharing a common dominant genus. This work provides a baseline for future studies on the impact of climate change on the microbial biodiversity of temperate forests in northern latitudes, and contributes to a growing collection of fermentative yeasts isolated from the wild with potential for biotechnological applications.