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bioRxiv · 10.1101/2025.01.20.633898

OT-Mation: an open-source code for parsing CSV files into python scripts for control of OT-2 liquid handling robotics

Laverick, A.·Convey, K.·Harrison, C.·Tomlinson, J. A.·Stach, J.·Howard, T. P.
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Abstract

OT-Mation is an open-source Python script designed to automate the programming of OT-2 liquid handling robots, making combinatorial experiments more accessible to researchers. By parsing user-defined CSV files containing information on labware, reagents, pipettes, and experimental design, OT-Mation generates a bespoke Python script compatible with the OT-2 system. OT-Mation enhances reproducibility, reduces human error, and streamlines workflows, making it a valuable addition to any laboratory utilising OT-2 robotics for liquid handling. While OT-Mation can be used for setting up any type of experiment on the OT-2, its real utility lies in making the connection between multifactorial experimental design software outputs (i.e. Design of Experiments arrays) and liquid-handling robot executable code. As such, OT-Mation helps bridge the gap between code-based flexibility and user-friendly operation, allowing researchers with limited programming skills to design and execute complex experiments efficiently.

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Laverick, A., Convey, K., Harrison, C., Tomlinson, J. A., Stach, J., Howard, T. P.. 2025-01-21. OT-Mation: an open-source code for parsing CSV files into python scripts for control of OT-2 liquid handling robotics. https://doi.org/10.1101/2025.01.20.633898

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