Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections

Abstract

Scanning fluorescence X-ray microscopy lets one non-destructively and quantitatively map the distribution of most biologically-important metals in cells and tissues. For studies on large-scale tissues and organs, a spatial resolution of several micrometers is often sufficient; in this case, bending magnets at synchrotron light sources provide abundant X-ray flux. We describe here the use of bending magnet beamline 8-BM-B at the Advanced Photon Source (APS) with two distinct microscopy stations: a pre-existing one with Kirkpatrick-Baez (KB) mirror optics for slightly higher throughput and the ability to accommodate samples tens of centimeters across, and a new prototype station with an axially-symmetric, single-bounce, capillary optic with slightly less flux, but slightly higher fluence (which affects achievable resolution at low metal concentration) and higher spatial resolution. The KB station provides{delta} res = 10.5 {micro}m spatial resolution at a per-pixel exposure time of tdwell = 100 ms and a fluence per time of 5.8x 107 photons /({micro}m2 {middle dot}s), while the prototype capillary station provides{delta} res = 6.3 {micro}m at tdwell = 50 ms and a fluence per time of 6.1x 107 photons ({micro}m2 {middle dot}s). We used image power spectral density to estimate the achieved spatial resolution{delta} res from individually acquired images, with{delta} res depending-on the optic, the fluorescence signal strength of the sample being imaged, and the method used to process raw fluorescence spectral data.