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bioRxiv · 10.1101/2025.01.08.631993

APMAT analysis reveals the association between CD8 T cell receptors, cognate antigen, and T cell phenotype and persistence

Abstract

Elucidating the relationships between a class I peptide antigen, a CD8 T cell receptor (TCR) specific to that antigen, and the T cell phenotype that emerges following antigen stimulation, remains a mostly unsolved problem, largely due to the lack of large data sets that can be mined to resolve such relationships. Here, we describe Antigen-TCR Pairing and Multiomic Analysis of T-cells (APMAT), an integrated experimental-computational framework designed for the high-throughput capture and analysis of CD8 T cells, with paired antigen, TCR sequence, and single-cell transcriptome. Starting with 951 putative antigens representing a comprehensive survey of the SARS-CoV-2 viral proteome, we utilize APMAT for the capture and single cell analysis of CD8 T cells from 62 HLA A*02:01 COVID-19 participants. We leverage this unique, comprehensive dataset to integrate with peptide antigen properties, TCR CDR3 sequences, and T cell phenotypes to show that distinct physicochemical features of the antigen-TCR pairs strongly associate with both T cell phenotype and T cell persistence. This analysis suggests that CD8+ T cell phenotype following antigen stimulation is at least partially deterministic, rather than the result of stochastic biological properties.

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Xie, J., Chen, D. G., Chour, W., Ng, R. H., Zhang, R., Yuan, D., Choi, J., McKasson, M., Troisch, P., Smith, B., Jones, L., Webster, A., Rasheed, Y., Li, S., Edmark, R., Hong, S., Murray, K. M., Logue, J. K., Franko, N. M., Lausted, C. G., Piening, B., Algren, H., Wallick, J., Magis, A. T., Watanabe, K., Mease, P., Greenberg, P. D., Chu, H., Goldman, J. D., Su, Y., Heath, J. R.. 2025-01-09. APMAT analysis reveals the association between CD8 T cell receptors, cognate antigen, and T cell phenotype and persistence. https://doi.org/10.1101/2025.01.08.631993

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