A 3D-printed cradle for mouse preclinical MRI with an integrated water heating system

Gaudin, R.; Bernard, J.; Glatigny, M.; Boido, D.

doi:10.1101/2024.12.29.630663

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bioRxiv · 10.1101/2024.12.29.630663

A 3D-printed cradle for mouse preclinical MRI with an integrated water heating system

Gaudin, R.·Bernard, J.·Glatigny, M.·Boido, D.

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Abstract

Functional Magnetic Resonance Imaging (fMRI) of small animals is mainly performed under sedation or anesthesia to avoid movement, which is detrimental to image quality. Heating systems to warm the animals usually rely on airflow or heating blankets or pads with circulating water to comply with MR compatibility requirements. However, these solutions are often suboptimal for small animals like mice scanned at ultra-high magnetic fields with long-bore MR scanners. We designed and built an MR cradle with an integrated water chamber, maximizing the contact surface with the mouses body. This large contact surface helps maintain body temperature without overheating the animal, thus reducing the risk of burns and hyperthermia. Our cradle keeps the mouses body temperature stable within the physiological range during an MRI session and fits the bore of a Bruker 17.2T scanner. We share the 3D drawings and all the information needed to replicate the cradle. Our design can be adapted to work on preclinical scanners with similar bore sizes and customized to add stimulation devices.

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Gaudin, R., Bernard, J., Glatigny, M., Boido, D.. 2024-12-30. A 3D-printed cradle for mouse preclinical MRI with an integrated water heating system. https://doi.org/10.1101/2024.12.29.630663

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