CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

Di Sante, M.; Pezzotti, M.; Zimmermann, J.; Enrico, A.; Deschamps, J.; Balmas, E.; Becca, S.; Reali, A.; Bertero, A.; Jug, F.; Pasqualini, F.

doi:10.1101/2024.12.19.629259

bioRxiv · 10.1101/2024.12.19.629259

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

Di Sante, M.·Pezzotti, M.·Zimmermann, J.·Enrico, A.·Deschamps, J.·Balmas, E.·Becca, S.·Reali, A.·Bertero, A.·Jug, F.·Pasqualini, F.

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Abstract

Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.

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Di Sante, M., Pezzotti, M., Zimmermann, J., Enrico, A., Deschamps, J., Balmas, E., Becca, S., Reali, A., Bertero, A., Jug, F., Pasqualini, F.. 2024-12-22. CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies. https://doi.org/10.1101/2024.12.19.629259

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