Bacteria-powered living materials enable coral larval settlement

Levy, N.; Kundu, S.; Freckelton, M. L.; Dinasquet, J.; Flores, I.; Galindo-Martinez, C. T.; Tresguerres, M.; De La Garza, V.; Sun, Y.; Karimi, Z.; Drury, C.; Jury, C. P.; Hancock, J.; Chen, S.; Hadfield, M. G.; R3D Consortium,; Wangpraseurt, D.

doi:10.1101/2024.12.18.629188

bioRxiv · 10.1101/2024.12.18.629188

Bacteria-powered living materials enable coral larval settlement

Levy, N.·Kundu, S.·Freckelton, M. L.·Dinasquet, J.·Flores, I.·Galindo-Martinez, C. T.·Tresguerres, M.·De La Garza, V.·Sun, Y.·Karimi, Z.·Drury, C.·Jury, C. P.·Hancock, J.·Chen, S.·Hadfield, M. G.·R3D Consortium,·Wangpraseurt, D.

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Abstract

The global decline of coral reefs calls for new strategies to rapidly restock coral populations and maintain ecosystem functions and services. Low recruitment success on degraded reefs hampers coral sexual propagation and contributes to limited genetic diversity and reef resilience. Here, we introduce a living bacteria-powered reef ink (Brink) for assisted coral recruitment. Brink can be rapidly applied to restoration substrates via photopolymerization, and it has been formulated to cultivate two settlement-inducing bacterial strains (Cellulophaga lytica and Thalassotalea euphylliae). Settlement assays performed with broadcast spawning (Montipora capitata) and brooding (Pocillopora acuta) Indo-Pacific corals showed that Brink-coated substrates increased settlement >5-fold compared to uncoated control substrates. Brink can be applied as a coating or 3D bioprinted, leading to various potential applications for integration with reef engineering. Our approach underscores the potential of using functional living materials for augmented ecosystem engineering and reef rehabilitation. SynopsisThis study introduces a functional and sustainable bacteria-powered living material that enhances coral settlement, promoting coral reef rehabilitation and ecosystem resilience.

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Levy, N., Kundu, S., Freckelton, M. L., Dinasquet, J., Flores, I., Galindo-Martinez, C. T., Tresguerres, M., De La Garza, V., Sun, Y., Karimi, Z., Drury, C., Jury, C. P., Hancock, J., Chen, S., Hadfield, M. G., R3D Consortium,, Wangpraseurt, D.. 2024-12-21. Bacteria-powered living materials enable coral larval settlement. https://doi.org/10.1101/2024.12.18.629188

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