Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology

Passmore, J.; Rates, A.; Schroeder, J.; Van Laarhoven, M.; Hellebrekers, V.; Van Hoef, H.; Geurts, A.; Van Straaten, W.; Nijenhuis, W.; Berger, F.; Smith, C.; Smal, I.; Kapitein, L.

doi:10.1101/2024.12.12.628240

bioRxiv · 10.1101/2024.12.12.628240

Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology

Passmore, J.·Rates, A.·Schroeder, J.·Van Laarhoven, M.·Hellebrekers, V.·Van Hoef, H.·Geurts, A.·Van Straaten, W.·Nijenhuis, W.·Berger, F.·Smith, C.·Smal, I.·Kapitein, L.

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Abstract

Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present outcome-driven microscopy, which combines smart microscopy with optogenetics to achieve subcellular spatiotemporal control of biology to predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, and demonstrate unprecedented spatiotemporal control over cellular behaviour.

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Passmore, J., Rates, A., Schroeder, J., Van Laarhoven, M., Hellebrekers, V., Van Hoef, H., Geurts, A., Van Straaten, W., Nijenhuis, W., Berger, F., Smith, C., Smal, I., Kapitein, L.. 2024-12-17. Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology. https://doi.org/10.1101/2024.12.12.628240

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